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mouse cd4 t cells  (R&D Systems)


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    R&D Systems mouse cd4 t cells
    Mouse Cd4 T Cells, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/magcellect+mouse+naive+cd4+t+cell+isolation+kit/MagCellect+Mouse+Naive+CD4%2B+T+Cell+Isolation+Kit/10__3389_slash_fimmu__2025__1700154-78-0-16
    Average 94 stars, based on 30 article reviews
    mouse cd4 t cells - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Role of Wnt3a expressed by dendritic cells in the activation of canonical Wnt signaling and generation of memory T cells during primary immune responses.
    Article Snippet: The presence of memory T cells (TMs) hinders transplant survival.. Dendritic cells (DCs) induce the generation of TMs during primary immune responses.. However, the specific mechanisms are unclear.

    Isolation:

    Article Title: Role of Wnt3a expressed by dendritic cells in the activation of canonical Wnt signaling and generation of memory T cells during primary immune responses.
    Article Snippet: The presence of memory T cells (TMs) hinders transplant survival.. Dendritic cells (DCs) induce the generation of TMs during primary immune responses.. However, the specific mechanisms are unclear.

    Article Title: Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation
    Article Snippet: .. CD4 + naive T cells were magnetically isolated from splenocyte suspensions with the MagCellect Mouse Naive CD4 + T Cell Isolation kit (R&D Systems). .. T cells were labeled with CFSE (Life Technologies) and co-cultured with eosinophils for 4 d at a 1:1 ratio in the presence of 30 U/ml recombinant human IL-2 (Proleukin, Prometheus), 10 ng/ml IL-5 and mouse T-Activator CD3/CD28 Dynabeads (Life Technologies).

    Article Title: Enhanced susceptibility to chemically induced colitis caused by excessive endosomal TLR signaling in LRBA-deficient mice
    Article Snippet: .. Briefly, naive CD4 + T cells were isolated from spleens by negative selection with the Naive CD4 + T Cell Isolation Kit (Miltenyi Biotec) or MagCellect Mouse Naive CD4 + T Cell Isolation Kit (R&D Systems). .. Cells were then stained with anti-CD4 (RM4-5), anti-CD45RB (C363-16A), anti-CD25 (PC61), anti-CD44 (IM7), and CD4 + CD25 − CD44 low CD45RB hi cells were sorted with a FACSAria II cell sorter (BD Biosciences).

    Article Title: Enhanced susceptibility to chemically induced colitis caused by excessive endosomal TLR signaling in LRBA-deficient mice.
    Article Snippet: .. Briefly, naive CD4+ T cells were isolated from spleens by negative selection with the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec) or MagCellect Mouse Naive CD4+ T Cell Isolation Kit (R&D Systems). .. Cells were then stained with anti-CD4 (RM4-5), anti-CD45RB (C36316A), anti-CD25 (PC61), anti-CD44 (IM7), and CD4+CD25−CD44lowCD45RBhi cells were sorted with a FACSAria II cell sorter (BD Biosciences).

    Article Title: An Antibiotic-Impacted Microbiota Compromises the Development of Colonic Regulatory T Cells and Predisposes to Dysregulated Immune Responses
    Article Snippet: For DC/T-cell cocultures, CD11c + DCs were isolated by positive selection from MLN single-cell suspensions using CD11c ultrapure mouse microbeads (Miltenyi Biotech). .. Naive CD4 + T cells were isolated by negative selection from splenic single-cell suspensions using the MagCellect mouse naive CD4 + T-cell isolation kit (R&D Systems). .. DCs and T cells were cocultured at 1:5 ratios (20,000 DCs and 100,000 T cells) in a round-bottom 96-well plate for 72 h in the presence of anti-CD3 agonistic antibody (BD Bioscience) at a 1-μg/ml final concentration with 5 ng/ml of TGF-β1 and 10 ng/ml of IL-2 (both from R&D Systems).

    Article Title: Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation.
    Article Snippet: .. CD4+ naive T cells were magnetically isolated from splenocyte suspensions with the MagCellect Mouse Naive CD4+ T Cell Isolation kit (R&D Systems). .. T cells were labeled with CFSE (Life Technologies) and co-cultured with eosinophils for 4 d at a 1:1 ratio in the presence of 30 U/ml recombinant human IL-2 (Proleukin, Prometheus), 10 ng/ml IL-5 and mouse T-Activator CD3/CD28 Dynabeads (Life Technologies).

    Article Title: MicroRNA-21 regulates T-cell apoptosis by directly targeting the tumor suppressor gene Tipe2
    Article Snippet: .. Naive CD4+ T cells were isolated from the spleen of WT and Tipe2-deficient mice using MagCellect Mouse Naive CD4+ T Cell Isolation Kit (R&D Systems, Minneapolis, MN, USA). ..

    Selection:

    Article Title: Role of Wnt3a expressed by dendritic cells in the activation of canonical Wnt signaling and generation of memory T cells during primary immune responses.
    Article Snippet: The presence of memory T cells (TMs) hinders transplant survival.. Dendritic cells (DCs) induce the generation of TMs during primary immune responses.. However, the specific mechanisms are unclear.

    Article Title: Enhanced susceptibility to chemically induced colitis caused by excessive endosomal TLR signaling in LRBA-deficient mice
    Article Snippet: .. Briefly, naive CD4 + T cells were isolated from spleens by negative selection with the Naive CD4 + T Cell Isolation Kit (Miltenyi Biotec) or MagCellect Mouse Naive CD4 + T Cell Isolation Kit (R&D Systems). .. Cells were then stained with anti-CD4 (RM4-5), anti-CD45RB (C363-16A), anti-CD25 (PC61), anti-CD44 (IM7), and CD4 + CD25 − CD44 low CD45RB hi cells were sorted with a FACSAria II cell sorter (BD Biosciences).

    Article Title: Enhanced susceptibility to chemically induced colitis caused by excessive endosomal TLR signaling in LRBA-deficient mice.
    Article Snippet: .. Briefly, naive CD4+ T cells were isolated from spleens by negative selection with the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec) or MagCellect Mouse Naive CD4+ T Cell Isolation Kit (R&D Systems). .. Cells were then stained with anti-CD4 (RM4-5), anti-CD45RB (C36316A), anti-CD25 (PC61), anti-CD44 (IM7), and CD4+CD25−CD44lowCD45RBhi cells were sorted with a FACSAria II cell sorter (BD Biosciences).

    Article Title: An Antibiotic-Impacted Microbiota Compromises the Development of Colonic Regulatory T Cells and Predisposes to Dysregulated Immune Responses
    Article Snippet: For DC/T-cell cocultures, CD11c + DCs were isolated by positive selection from MLN single-cell suspensions using CD11c ultrapure mouse microbeads (Miltenyi Biotech). .. Naive CD4 + T cells were isolated by negative selection from splenic single-cell suspensions using the MagCellect mouse naive CD4 + T-cell isolation kit (R&D Systems). .. DCs and T cells were cocultured at 1:5 ratios (20,000 DCs and 100,000 T cells) in a round-bottom 96-well plate for 72 h in the presence of anti-CD3 agonistic antibody (BD Bioscience) at a 1-μg/ml final concentration with 5 ng/ml of TGF-β1 and 10 ng/ml of IL-2 (both from R&D Systems).

    Cell Isolation:

    Article Title: Role of Wnt3a expressed by dendritic cells in the activation of canonical Wnt signaling and generation of memory T cells during primary immune responses.
    Article Snippet: The presence of memory T cells (TMs) hinders transplant survival.. Dendritic cells (DCs) induce the generation of TMs during primary immune responses.. However, the specific mechanisms are unclear.

    Article Title: Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation
    Article Snippet: .. CD4 + naive T cells were magnetically isolated from splenocyte suspensions with the MagCellect Mouse Naive CD4 + T Cell Isolation kit (R&D Systems). .. T cells were labeled with CFSE (Life Technologies) and co-cultured with eosinophils for 4 d at a 1:1 ratio in the presence of 30 U/ml recombinant human IL-2 (Proleukin, Prometheus), 10 ng/ml IL-5 and mouse T-Activator CD3/CD28 Dynabeads (Life Technologies).

    Article Title: Enhanced susceptibility to chemically induced colitis caused by excessive endosomal TLR signaling in LRBA-deficient mice
    Article Snippet: .. Briefly, naive CD4 + T cells were isolated from spleens by negative selection with the Naive CD4 + T Cell Isolation Kit (Miltenyi Biotec) or MagCellect Mouse Naive CD4 + T Cell Isolation Kit (R&D Systems). .. Cells were then stained with anti-CD4 (RM4-5), anti-CD45RB (C363-16A), anti-CD25 (PC61), anti-CD44 (IM7), and CD4 + CD25 − CD44 low CD45RB hi cells were sorted with a FACSAria II cell sorter (BD Biosciences).

    Article Title: Enhanced susceptibility to chemically induced colitis caused by excessive endosomal TLR signaling in LRBA-deficient mice.
    Article Snippet: .. Briefly, naive CD4+ T cells were isolated from spleens by negative selection with the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec) or MagCellect Mouse Naive CD4+ T Cell Isolation Kit (R&D Systems). .. Cells were then stained with anti-CD4 (RM4-5), anti-CD45RB (C36316A), anti-CD25 (PC61), anti-CD44 (IM7), and CD4+CD25−CD44lowCD45RBhi cells were sorted with a FACSAria II cell sorter (BD Biosciences).

    Article Title: Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation.
    Article Snippet: .. CD4+ naive T cells were magnetically isolated from splenocyte suspensions with the MagCellect Mouse Naive CD4+ T Cell Isolation kit (R&D Systems). .. T cells were labeled with CFSE (Life Technologies) and co-cultured with eosinophils for 4 d at a 1:1 ratio in the presence of 30 U/ml recombinant human IL-2 (Proleukin, Prometheus), 10 ng/ml IL-5 and mouse T-Activator CD3/CD28 Dynabeads (Life Technologies).

    Article Title: MicroRNA-21 regulates T-cell apoptosis by directly targeting the tumor suppressor gene Tipe2
    Article Snippet: .. Naive CD4+ T cells were isolated from the spleen of WT and Tipe2-deficient mice using MagCellect Mouse Naive CD4+ T Cell Isolation Kit (R&D Systems, Minneapolis, MN, USA). ..

    Single Cell:

    Article Title: An Antibiotic-Impacted Microbiota Compromises the Development of Colonic Regulatory T Cells and Predisposes to Dysregulated Immune Responses
    Article Snippet: For DC/T-cell cocultures, CD11c + DCs were isolated by positive selection from MLN single-cell suspensions using CD11c ultrapure mouse microbeads (Miltenyi Biotech). .. Naive CD4 + T cells were isolated by negative selection from splenic single-cell suspensions using the MagCellect mouse naive CD4 + T-cell isolation kit (R&D Systems). .. DCs and T cells were cocultured at 1:5 ratios (20,000 DCs and 100,000 T cells) in a round-bottom 96-well plate for 72 h in the presence of anti-CD3 agonistic antibody (BD Bioscience) at a 1-μg/ml final concentration with 5 ng/ml of TGF-β1 and 10 ng/ml of IL-2 (both from R&D Systems).



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